Biochemical Testing Bacteria Species Based On Their...

Introduction: Biochemical tests are used to identify bacteria species based on their differences in their biochemical activities of different bacteria. To differentiate one bacteria from another, each species of bacteria has a well-defined set of metabolic activities. Bacteria can be differentiated in many ways. They can be based on structural differences or metabolic differences. Structurally, the Gram stain is the most fundamental technique in identifying bacterial species and was developed by Christian Gram in 1884. Gram negative bacteria can be distinguished from Gram positive with the presence of LPS, lipopolysaccharide and a thinner peptidoglycan layer which doesn’t retain crystal violet as well. For this experiment, an unknown bacterium was identified from a list of 32 clinically significant bacteria. It was first isolated onto a TSA plate and then through a series of differential and selective tests were performed to determine its identity to be Klebsiella ozaenae. Materials and Methods: Dichotomous Key Preparation: A tree diagram was constructed for the 32 clinically significant bacteria. From this tree diagram, a dichotomous key was created to identify the unknown provided by the instructor. Culture Isolation: A suspension containing mixture of an unknown bacteria was streaked on a Blood agar (BA) plate and incubated overnight at 35 °C for single colony isolation. Through streak plate isolation, a single colony of bacteria was picked up from the plate and streakedShow MoreRelatedGeneral Unknown . Introduction: . The General Unknown Lab1482 Words   |  6 PagesGeneral Unknown Introduction: The general unknown lab combines methods and ideas around biochemical and physiological activities of microorganisms for the identification of unknown pure culture samples of gram positive and negative bacteria. The purpose of using biochemical and physiological aspects of bacteria for testing and identification is used in everything from choosing an effective treatment for microbial-causing diseases to understanding the chemical reactions that occur within ourRead MoreUnknown Project Essay2242 Words   |  9 Pagesdetect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent incomplete hemolysis. A transparent media around the bacteria colony represents complete lysis of the red blood cells. If no change is observed around the bacteria colony thenRead MoreAntimicrobial Activity of Soil- Research Proposal Abstract2803 Words   |  12 PagesANTIMICROBIAL ACTIVITY OF SOIL BY SITUMBEKO LIWELEYA (s213459531) Research Proposal Submitted in fulfillment of the requirements for the degree of Bachelor of BIOMEDICAL TECHNOLOGY | | FACULTY OF HEALTH SCIENCES | at Nelson Mandela Metropolitan University | MODULE- RESEARCH METHODOLOGY, SUPERVISOR- PROFESSOR N. SMITH | Contents ANTIMICROBIAL PROPERTIES OF SOIL 2 ABSTRACT 2 LITERATURE REVIEW 3 AIM OF RESEARCH 4 OBJECTIVES OF STUDY 4 RESEARCH METHODOLOGY 4 Read MoreBacteria can be transferred to food from utensils that are not cleaned and sanitized properly. The2500 Words   |  10 PagesBacteria can be transferred to food from utensils that are not cleaned and sanitized properly. The most common factors in bacterial transfer from one surface to another are moisture, contact time and pressure which can result in higher transfer between surfaces. Since some bacteria or other pathogenic microorganisms can survive outside the human body under favourable conditions of considerable period of time, unsanitized utensils such as glass, spoons and forks could be a direct or indirect sourcesRead MoreMolecular And Phylogenetic Characterization Of Mercury Reductase Gene ( Mera ) From Newly Isolated Bacillus Sp2109 Words   |  9 Pagesmercury as pollutant into water bodies has arise the level of mercury from 20 to 100 times or even more from the permissible limit of it. The contamination has affected the marine population at large but few of bacteria have found way out to tolerate such high concentration of mercury. Such bacteria have been discovered way back in 1960’s by Moore, where he reported to found an operon called as mercury operon or mer operon which is found to be integrated on plasmid, transposons, etc., which harbors theRead MoreSafety of Genetically Modified Organisms1138 Words   |  5 Pagesright to know what I’ m buying and putting in my children’s mouths. First of all genetically Modified Organisms are plants or animals that have been genetically engineered with DNA from bacteria, viruses or other plants and animals. These experimental combinations of genes from different species cannot occur in nature or in traditional crossbreeding. The biotech companies claim that Genetically Modified crops, also known as GMOs are safe to eat. Yet there are no long- term studiesRead MoreIndustrial Attachment Report at Cmr-Kemri9715 Words   |  39 PagesUniversity of Science and Technology(MMUST).This attachment is purposely meant to bring into practice the knowledge and skills gained in classroom and also enable me to understand practical working methodologies in different companies/institutions.This report serves to summarize the activities and experiences gained with CMR-KEMRI for the period of three months. Objectives: –To have an opportunity to apply the principles and techniques theoretically learnt into real-life problemRead MoreMarine Extract Molecules And Their Application Essay2092 Words   |  9 PagesMarine extract molecules and their application in inhibiting E.coli quorum sensing as a potential antibacterial pharmaceutical Abstract Quorum sensing, or cell to cell communication, is an occurrence that is very widespread amongst pathogenic bacteria which infect the human population. With increased development of bacterial resistance against antibiotics, scientists have taken an anti-bacterial approach to manipulate quorum sensing and impede cell growth and communication. Quorum sensing can beRead More The Impact of Human Activities on the Environment Essay1764 Words   |  8 PagesThe Impact of Human Activities on the Environment Everything that humans do has some impact on the environment. Pollution is a result of human activities in the environment. The main sources of pollution are farming, water pollution and air pollution. Farming is having an increased impact on the environment. This is because there is increased pressure on farmers to produce a single crop and to concentrate on one form of animal husbandry, such as, dairy cattle or pigRead MoreMango (Mangifera Indica) Leaves Extract and Coconut Oil as an Antibacterial Ointment4132 Words   |  17 PagesBureau of Plant Industry. It was tested against the bacteria E. coli and S. aureus. The resulting Numerical value was 2.5 for E. coli and 3.0 for S. aureus. The bureau used the standard parameter 1-2- slightly inhibited and 3-5 as partially inhibited. Thus, the inhibition of the mango leaves extract and coconut oil in E. coli was slight and it is partial in S. aureus. The researcher concluded that mango leaves extract with coconut oil can kill bacteria like E. coli and S. aureus. It can also be concluded